Acid Phosphatases from the Liver of Labeo rohita: Purification and Characterization
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چکیده
منابع مشابه
Acid phosphatases from the liver of Labeo rohita: purification and characterization.
Low molecular weight acid phosphatase (LM-ACP) peak 2 (the isoenzyme corresponding to isoform 2, IF-2) from the liver of fish Rahu (Labeo rohita) was purified to homogeneity. 900 times purification resulted with specific activity of 35 U/mg of protein and recovery of 0.2%. The enzyme was found homogeneous on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Molecular weight...
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The maximum acid phosphatasic activity was detected in peanut seed at the 5th day of germination. At least, two acid phosphatases were purified by successive chromatography separations on DEAE-Sepharose CL-6B, CM-Sepharose CL-6B, Sephacryl S-100 HR, and Phenyl-Sepharose HP to apparent homogeneity from five days old cotyledon of peanut after germination. These isoenzymes, designated peanut cotyl...
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Interleukin-10, an important regulator of both the innate and adaptive immune systems, is a multifunctional major cytokine. Though it is one of the major cytokines, IL-10 from the Indian major carp, Labeo rohita, has not yet been characterized. In the present study, we report large scale production and purification of biologically active recombinant IL-10 of L. rohita (rLrIL-10) using a heterol...
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Degenerative histopathalogical alterations were seen in liver due to chronic exposure of the fish, Labeo rohita to chlorides of chromium, nickel and zinc for 30 days. However, more severe degenerative changes were observed in case of zinc chloride exposed fish than nickel chloride and chromium chloride treated fishes, indicating more toxic nature of zinc chloride. There were enlarged nuclei, co...
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Labeo rohita fingerlings were exposed to zinc metal toxicity (5 and 10 ppm) for duration of 5 and 15 days. The histological changes were studied in brain and liver of the treated fish. The brain tissues showed enlarged pyramidal cells with extensive vacoulation while severe necrosis, haemorrhage and degeneration of hepatocytes were witnessed in the liver tissues.
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2008
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.31.802